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Two of the N-glycosylation sites predicted for human LIPI at Asn63-Asn64-Ser65 and at Asn396-397Ile- 398Ile (designated as sites 3 and 9, respectively) were retained for most of the 11 mammalian LIPI sequences examined, although predicted N-glycolsylation sites were observed at other positions for some sequences, including Asn23-Thr24-Thr52 (site 1) for mouse LIPI

There, they are broken down through oxidation and converted to energy for all tissues, including the heart, liver, and skeletal muscles

Reads were further filtered using SAMTools to remove orphan reads (reads in which one mate in a pair does not align but the other does) 116

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Each mouse was placed adjacent to the doorway and allowed to explore for 10 min